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KMID : 0545120070170020335
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Journal of Microbiology and Biotechnology
2007 Volume.17 No. 2 p.335 ~ p.341
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Qualitative and Quantitative Analysis of Genetically Modified Pepper
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Song Hee-Sung
Kim Jae-Hwan
Kim Dong-Hern
Kim Hae-Yeong
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Abstract
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For the development of qualitative and quantitative PCR methods of genetically modified (GM) pepper developed in Korea, a capsanthin-capsorubin synthase (CCS) gene was used as the endogenous reference gene. The primer pair ccs- F/R amplifying the pepper endogenous gene gave rise to an amplicon of 102 bp. No amplified product was observed when DNA samples from 16 different plants were used as templates. The construct-specific primer pairs amplifying the junction region of the bar gene and Ti7 introduced in GM pepper gave rise to an amplicon of 182 bp. Quantitative PCR assay was performed using a TaqMan probe and a standard plasmid as a reference molecule, which contained both an endogenous and event-specific sequence. For the validation of this method, the test samples containing 0.1, 1, 3, 5, and 10% GM pepper were quantified.
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KEYWORD
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Capsanthin-capsorubin synthase, GMO detection, GM pepper, real-time PCR
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